Description
eDNA filters are a useful tool when testing surface water bodies as to the presence of certain organisms. Perhaps an invasive species has been noted and one wants to determine its presence. Surface water bodies such a ponds, streams and lakes can be sampled using Waterra eDNA filters by pumping water from that body through the filter. DNA in the water is captured by the filter and can be preserved for extraction in a laboratory setting.
The “e” in eDNA stands for Environmental and “DNA” is Deoxyribonucleic Acid, well known to be the building block of life. DNA is contained in every living organism’s cell and is shed into the environment in many ways. The organism may live or lived in the water body or catchment area, was passing through or was transported in. Irrespective of how it got there, if it is in the water there is a chance that the DNA could be in the water sample processed through the eDNA filter.
An advantage of using Waterra eDNA filters is that the large surface area of the filter (either 300 cm² or 600 cm²) allows large volumes of water to be run through. Samples up to 100 liters or more, depending on the turbidity of the water increase the odds of capturing eDNA of the target species. Use of a pump such as a Peristaltic Pump in association with the eDNA filter, can facilitate this large sample volume.
Very straightforward to use. Simply plug a Waterra eDNA filter into your suction pump tubing and place the filter inlet end into the water body to be sampled. Run the designated volume of water through the inline disposable filter capturing particulate matter including eDNA in the filter.
A buffer solution is typically added to preserve the eDNA. Fit an end cap tightly on the inlet side. Fill the filter with the desired buffer to preserve the DNA and fit the second end cap to trap the Buffer in the filter. The Waterra end plugs retain the buffer and eDNA for further processing. End plugs are provided in each sealed eDNA filter bag.
eDNA Syringe Adapters are used in conjunction with eDNA 100 ml Syringes to inject and extract buffer from the eDNA Filters. eDNA filter adapters must only be used on one sample to prevent cross contamination. One syringe will service numerous filters when used with eDNA Syringe Adapters.
The filter accommodates 3/8″ ID and 1/2″ ID and 1/4″ OD tubing on the inlet and 1/2″ ID and 1/4″ OD tubing on the outlet.
Waterra eDNA filters are PFAS, PFOS & PFOA free.