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Microbial Insights CENSUS® (qPCR) – Environmental Remediation

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Microbial Insights CENSUS® (qPCR) – Environmental Remediation

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CENSUS employs a revolutionary molecular biological tool (MBT) called quantitative polymerase chain reaction (qPCR) for enumeration of specific organisms or genes encoding specific biological functions.

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Description

CENSUS employs a revolutionary molecular biological tool (MBT) called quantitative polymerase chain reaction (qPCR) for enumeration of specific organisms or genes encoding specific biological functions. While used in many fields, qPCR or CENSUS has been most frequently applied to the environmental restoration industry to allow site managers to cost-effectively detect and quantify targeted members of the microbial community deemed critical for bioremediation. At a site impacted by chlorinated solvents like PCE or TCE for example, CENSUS quantification of Dehalococcoides, a key group of dechlorinating bacteria, permits project managers to address the following:

– evaluate the feasibility of monitored natural attenuation (MNA)
– evaluate the efficacy of enhanced bio-remediation approaches
– assess the need for bio-augmentation.

Application of the CENSUS technology, however, is not limited to evaluation of biodegradation of chlorinated solvents. Currently, MI offers over 50 CENSUS assays targeting a broad spectrum of microbial functions that can provide direct evidence of the biological processes occurring at your site.

Additional information

Quantifys

Dehalobacter), Specific microorganisms (e.g.?Dehalococcoides

Analysis

qPCR

Functionality

Sampling

Available for Rent

No

Supplier

Microbial Insights

Datasheet

Manual

Additional Documents


Microbial Sampling and laboratory characterisation 

Microbial Insights (MI) is an environmental biotechnology laboratory specializing in the development and application of cutting edge molecular biological tools to describe and quantify microbial populations. Since 1992, MI has become a leader in the application of nucleic acid (DNA and RNA) and lipid biomarkers (PLFA) for more effective evaluation of microbial processes ranging from bioremediation to microbiologically influenced corrosion.

Microbial Insights Australia was launched in 2013 to better serve our clients and offers a full spectrum of molecular biological tools including qPCR, QuantArray®, In Situ Microcosms (ISMs) and Stable Isotope Probing (SIP). Sample preparation and extractions are performed upon receipt at the MI Australia laboratory just outside of Adelaide eliminating international shipping.

Flagship Tools:

1. CENSUS® qPCR

Quantitative polymerase chain reaction is a DNA based molecular biological tool for quantification of specific target genes or microorganisms present in an environmental sample.

2. QuantArray

QuantArray is a hybrid technology combining the highly parallel detection of DNA microarrays with the accurate and precise quantification of qPCR into a single platform. For more comprehensive evaluation of biodegradation, use QuantArray to simultaneously and cost-effectively quantify an entire suite of key contaminant degrading microorganisms and functional genes.

3. In Situ Microcosms

Site managers have frequently turned to laboratory microcosms or pilot studies to evaluate bioremediation. However, duplication of in situ conditions in the laboratory is difficult and the results often do not correlate to the field. Pilot studies are often prohibitively expensive as an investigative tool. In Situ Microcosm studies serve as cost-effective, in situ microcosms providing microbial, chemical, and geochemical evidence to screen remedial alternatives.


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