Description
Microbial Insights Stable Isotope Probing (SIP) is an innovative MBT that uses a Bio-Trap® amended with a 13C “labelled” contaminant (e.g. 13C benzene) to conclusively determine whether biodegradation has occurred. The 13C label serves much like a tracer which can be detected in the end products of biodegradation – microbial biomass and CO2 .
CONCLUSIVE With the SIP method, a Bio-Trap® amended with a 13C labeled contaminant is deployed in an impacted monitoring well for 30 to 60 days. Detection of 13C enriched phospholipid fatty acids (PLFA) following in field deployment, conclusively demonstrates in situ biodegradation and incorporation into microbial biomass. Detection of 13C enriched dissolved inorganic carbon demonstrates contaminant mineralization.
VERSATILE SIP can provide conclusive evidence of biodegradation of many common contaminants including benzene, other BTEX compounds, MTBE, naphthalene, and other contaminants that microorganisms can utilize as carbon and energy sources.
COST EFFECTIVE SIP ultimately saves money by allowing site managers and regulators to make more informed decisions. SIP is often employed during remedy selection to conclusively evaluate in situ biodegradation of a key contaminant and the feasibility of monitored natural attenuation (MNA).
IN SITU
Unlike laboratory studies, SIP study results reflect in situ biodegradation activity because the 13C contaminant adsorbed to the Bio-Trap® is exposed to the same subsurface conditions and processes as the site contaminants during the field deployment period.
